Wazir Agha Library, UOS

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DNA sequencing III :

Additional authors: Kieleczawa, Jan.
Published by : Jones and Bartlett Publishers, (Sudbury, Mass. :) Physical details: xvi, 184 p., 8 p. of plates : ill. (some col.) ; 24 cm. ISBN:9780763742973 (alk. paper); 076374297X (alk. paper).
Subject(s): DNA -- Analysis. | Nucleotide sequence -- Methodology. | Sequence Analysis, DNA -- methods. | Base Sequence. | Templates, Genetic.
Year: 2009
Online resources:
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Item type Current location Call number Status Date due Barcode
University of Sargodha-Central Library
572.86 KID (Browse shelf) Available 91235

Includes bibliographical references and index.

Controlled heat-denaturation of DNA plasmids and PCR fragments -- Heat denaturation is an effective step in sequencing of many difficult DNA templates -- Solutions for sequencing difficult regions -- Improving sequence results from difficult templates with Phi 29 DNA polymerase and nucleotide analogs: the TempliPhi sequence resolver kit -- Sequencing through various secondary structures: detailed studies of pDEST vectors and other DNA templates with hairpins -- Transcriptional sequencing as a tool for reading difficult-to-read templates -- Bias-free cloning of "unclonable" DNA for simplified genomic finishing -- Bioinformatics tools to aide sequencing of difficult templates.

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