Amyloid proteins : methods and protocols / edited by Einar M. Sigurdsson - New York : Humana Press, c2005. - xiv, 392 p. : ill. ;vol;299 27 cm. - Methods in molecular biology ; v. 299 Methods in molecular biology .

Includes bibliographical references and index.

Rapid generation of dityrosine cross-linked A-[beta] oligomers via cu-redox cycling / Application of photochemical cross-linking to the study of oligomerization of amyloidogenic proteins / Preparation of stable amyloid b-protein oligomers of defined assembly order / Purification and fibrillation of full-length recombinant PrP / Featuring amyloids with fourier transform infrared and circular dichroism spectroscopies / Quasielastic light scattering study of amyloid b-protein fibrillogenesis / Conformations of microtubule-associated protein tau mapped by fluorescence resonance energy transfer / Measuring the kinetics of amyloid fibril elongation using quartz crystal microbalances / X-ray fibre diffraction studies of amyloid fibrils / Structural characterization of prefibrillar intermediates and amyloid fibrils by small-angle x-ray scattering / Atomic force fluorescence microscopy in the characterization of amyloid fibril assembly and oligomeric intermediates / Investigating fibrillar aggregates of tau protein by atomic force microscopy / Structural studies of amyloids by quenched hydrogen-deuterium exchange by NMR / Cyclic amplification of prion protein misfolding / Search for amyloid-binding proteins by affinity chromatography / Establishing the links between A[beta] aggregation and cytotoxicity in vitro using biophysical approaches / Preparation of cultured human vascular cells / Murine cerebrovascular cells as a cell culture model for cerebral amyloid angiopathy : isolation of smooth muscle and endothelial cells from mouse brain / In vitro assays measuring protection by proteins such as cystatin C of primary cortical neuronal and smooth muscle cells / Study of neurotoxic intracellular calcium signalling triggered by amyloids / Bacterial amyloids / Study of amyloids using yeast / Cell-to-cell transmission of [alpha]-synuclein aggregates / Subcutaneous adipose tissue biopsy for amyloid protein studies / Analysis of s100 oligomers and amyloids / S100A8/A9 amyloidosis in the ageing prostate : relating ex vivo and in vitro studies / Isolation of amyloid by solubilization in water / Histological staining of amyloid and pre-amyloid peptides and proteins in mouse tissue / Pentameric luminescent-conjugated oligothiophene for optical imaging of in vitro-formed amyloid fibrils and protein aggregates in tissue sections / In vivo magnetic resonance imaging of amyloid-[beta] plaques in mice / Mouse model for scrapie : inoculation, clinical scoring, and histopathological techniques / Biochemical isolation of insoluble tau in transgenic mouse models of tauopathies / Tissue processing prior to analysis of alzheimer's disease associated proteins and metabolites, including A[beta] / A-[beta] measurement by enzyme-linked immunosorbent assay / Cognitive and sensorimotor tasks for assessing functional impairments in mouse models of Alzheimer's Disease and related disorders / Adam P. Gunn, Blaine R. Roberts, and Ashley I. Bush -- Dahabada H.J. Lopes ... [et al.] -- Clark Rosensweig ... [et al.] -- Natallia Makarava and Ilia V. Baskakov -- Miguel Calero and María Gasset -- Aleksey Lomakin and David B. Teplow -- Sadasivam Jeganathan ... [et al.] -- Alexander K. Buell, Christopher M. Dobson, and Mark E. Welland -- Kyle L. Morris and Louise C. Serpell -- Annette Eva Langkilde and Bente Vestergaard -- Valeriy Ostapchenko, Maria Gasset, and Ilia V. Baskakov -- Susanne Wegmann, Daniel J. Muller, and Eckhard Mandelkow -- Marçal Vilar, Lei Wang, and Roland Riek -- Marcelo A. Barria, Dennisse Gonzalez-Romero, and Claudio Soto -- Miguel Calero, Agueda Rostagno, and Jorge Ghiso -- Asad Jan and Hilal A. Lashuel -- Ingvar H. Olafsson, Dadi Th. Vilhjalmsson, and Finnbogi R. Thormodsson -- Sebastien A. Gauthier ... [et al.] -- Sebastien A. Gauthier ... [ et al.] -- Carlos Villalobos ... [et al.] -- Yizhou Zhou ... [et al.] -- Reed B. Wickner ... [et al.] -- Seung-Jae Lee ... [et al] -- Per Westermark -- Hugo M. Botelho, Günter Fritz, and Cláudio M. Gomes -- Anna L. Gharibyan, Dina Raveh, and Ludmilla A. Morozova-Roche -- Dadi Th. Vilhjalmsson, Indiana E. Ingolfsdottir, and Finnbogi R. Thormodsson -- Hameetha B. Rajamohamedsait and Einar M. Sigurdsson -- K. Peter R. Nilsson, Mikael Lindgren, and Per Hammarström -- Youssef Zaim Wadghiri ... [et al.] -- Michele A. Di Bari, Romolo Nonno, and Umberto Agrimi -- Carl Julien, Alexis Bretteville, and Emmanuel Planel -- Stephen D. Schmidt, Ralph A. Nixon, and Paul M. Mathews -- Stephen D. Schmidt ... [et. al] -- Allal Boutajangout ... [et al.].

9781617795503 (alk. paper) 161779550X (alk. paper) 9781617795510 (e-ISBN) 1617795518 (e-ISBN)

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Amyloid--Laboratory manuals.
Amyloid beta-protein--Laboratory manuals.
Amyloid--biosynthesis--Laboratory Manuals.
Amyloid--isolation & purification--Laboratory Manuals.
Biological Assay--methods--Laboratory Manuals.
Cells, Cultured--cytology--Laboratory Manuals.
Amyloid.
Amyloid beta-protein.


Handbooks, manuals, etc.

552.45 / A495 2012

571.6/4078